Ettan ipgphor user manual

Ettan ipgphor 3 isoelectric focusing unit cytiva formerly. Whatman 80649857 ipgphor manifold ceramic tray for use. Data file 28404198 aa ettan ipgphor 3 ettan ipgphor 3. Ettan ipgphor 3 ief system from ge healthcare used ettan ipgphor 3 isoelectric focusing unit. Ettan ipgphor 3 manifold is an accessory for firstdimension ief of proteins on. The ettan ipgphor cup loading manifold user manual contains instructions for firstdimension isoelectric focusing of proteins on ipg strips.

This system can run up to 12 ipg strips from 7cm to 24 cm. Page 1 ge healthcare ettan daltsix electrophoresis system user manual page 3 12 psig. Detailed protocols for gel casting can be found in the ettan dige user manual, ettan dalttwelve user manual, and ettan daltsix user manual. Ettan ipgphor 3 control software user manual 11003459 ettan ipgphor 3 instrument user manual 11003458 ettan ipgphor cup loading manifold user manual 11003460 2d electrophoresis principles and methods 80642960 ettan dalttwelve system user manual 80647653. For creating, editing, and running protocol programs from the ettan ipgphor control panel, please refer to the ettan ipgphor 3 instrument user manual. Equilibrate the focused ipg strips in sds equilibration. Ge healthcare ettan ipgphor 3 isoelectric focusing unit available on gsava contract for federal government customers only. The ettan ipgphor ii from ge healthcare is the ideal option for obtaining perfectly resolved first dimensions of 2d gels.

For detailed instructions on installation and usage of this software, refer to the ettan ipgphor 3 control software user manual. Multiphor and ipgphor isoelectric focusing systems. The firstdimension isoelectric focusing ief was performed using an ettan ipgphor system ge healthcare, pittsburgh, usa for a total of 60 kvh at 20 c, specifically in five steps. Ettan ipgphor 3 ief system cytiva, formerly ge healthcare. Equilibrate the focused ipg strips in sds equilibration buffer plus 1. Ge healthcare manifold kit for ettan ipgphor 3 isoelectric. Ipgphor manifold light 11002688 routine applications. Reports can be created, saved, printed, and exported. A0 0603 ettan ipgphor cup loading manifold ettan ipgphor isoelectric focusing system. Ettan dige system users manual university of alberta. Many of the latest user and service documents are available in pdf format. Ettan ipgphor 3 control software generates professional graphical reports with a large user interface for programming and editing. Click the link below to enter the ge healthcare customer documentation portal. Ettan ipgphor 3 ief system cytiva, formerly ge healthcare life.

Ge healthcare ettan daltsix user manual pdf download. Regardless of the features used, the et312b offers superior performance when compared to any erotic electrostim product. The procedures for equilibrating strips, positioning them, and electrophoresis are identical to those for standard 2d analysis. Support documentation library support ge healthcare. Ettan daltsixgel caster and gradient maker ettan daltsixgel caster holds up to six 1. Ge healthcare ettan ipgphor 3 isoelectric focusing unit isoelectric. The ettan dalt ii system is designed to handle multiple large seconddimension gels in a simple, ef. Ief using ettan ipgphor 3 isoelectric focusing system and. Ettan ipgphor 3 ief system is a fully integrated isoelectric focusing system optimized to deliver speed and reliability in the first dimension of 2d electrophoresis. For detailed instructions on installation and usage of ettan ipgphor control software, and also on computer instrument connection, please refer to the ettan ipgphor software user manual. Ettan daltsix electrophoresis system user manual ge healthcare 80649249ac. User friendly ettan ipgphor 3 control software is a user.

Ettan ipgphor 3 control software fig 22, with an external personal computer windows connected via a serial port, can be used to control up to four ettan ipgphor 3 units simultaneously, each running a different set of run parameters. Twodimensional separation of protein samples sigmaaldrich. Ettan ipgphor 3 is a fully integrated isoelectric focusing ief system optimized to deliver high throughput, speed, reproducibility and high proteinloading capacity. To control multiple units from one computer, an optional junction box is necessary. In each compartment, electrodes produce the electric field to cause the. Ergotron workfits user manual pdf download manualslib. It is very important that the user is familiar with the driving technique and is able to operate the controls competently before venturing far from where there is assistance. It is also compatible with the firstgeneration ettan ipgphor see ettan ipgphor user manual. Imaging twodimensional protein gels stained with sypro ruby. Ettan ipgphor3 ief system 7cm strip protocols youtube. The advanced topics section is for more advanced users. Data file 28937442 aa 2d electrophoresis ipgbox and ipgbox kit. Programmable functions include rehydration time, platform. Ettan ipgphor ii isoelectric focusing system performs the first dimension of 2d gel electrophoresis, protein isoelectric focusing ief.

Data file 28404198 aa ettan ipgphor 3 ettan ipgphor 3 isoelectric focusing system the ettan ipgphor 3 is a fully integrated isoelectric focusing ief system optimized to deliver high throughput, ease of use, speed, reproducibility, and high proteinloading capacity. Connect only to coolant sources with regulated pressure. For the latest user installation guide please visit. It is a powerful tool for separating complex mixtures of proteins by charge and size and for scanning and analyzing the resulting second. Ettan ipgphor 3 isoelectric focusing unit 1 11003364 ipgphor manifold ceramic tray 1 80649857 electrode set 1 80649876 sample cups 120 80649895 ipgphor strip holder cleaning solution 950 ml 80645278 dalt gel 12.

Mar 14, 2007 the ettan ipgphor ii from ge healthcare is the ideal option for obtaining perfectly resolved first dimensions of 2d gels. Ief using ettan ipgphor 3 isoelectric focusing system. To test the cell surface protein labeling protocol in an ettan dige experiment using all three dyes 6, a series of samples from serum depleted cho cells were collected and cell surface proteins labeled at different time points table 1. Ettan ipgphor 3 operating instructions 28963551 ac 5 operation 5. Ipgphor isoelectric focusing system user manual control panel the ipgphor is controlled by a 7key membrane keypad. A00603 ettan ipgphor ii isoelectric focusing system. User friendly ettan ipgphor 3 control software is a userfriendly interface to the ettan ipgphor. Ettan ipgphor 3 control software user manual 11003459 edition ab 7 1introduction 1. Ettan ipgphor 3 control software can be used to control up to four ettan ipgphor 3 units at one time, each running a different program. Ettan ipgphor ii isoelectric focusing system amersham. If fewer gels are to be cast, the unused volume is.

The default location of the cursor is always under the protocol. Ettan ipgphor 3 control software allows easy programming and viewing of run data. Divided into compartments with a platform in the middle, the horizontal electrophoresis systems completely submerge plated gel platforms in a buffer. Ettan ipgphor 3 isoelectric focusing system, ge healthcare. The user can create, save, and edit protocols and also pause during a run to edit a protocol in real time. Once in the portal, you can further narrow your search by selecting modality, product and document type using the integrated search tool. Ettan ipgphor manifold, complete includes 1 x cleaning brush, 2 x electrode assembly, 1 x spirit level, 20 x sample cup, 1 x sample cup insertion tool, 60 x paper electrode wicks, 60 x paper bridge pads, 1 x manifold, 1 x forceps, 1 x 60 ml cleaning solution and, user manual. Ettan dige system user manual 18117317 edition ab 9 1 introduction 1. Ettan ipgphor 3 ief system is a fully integrated isoelectric focusing system optimized to deliver speed and reliability in the first dimension of 2d electrophoresis run up to 12 ipg strips 7, 11, 18, or 24 cm simultaneously using ettan ipgphor manifold, ettan ipgphor manifold light, or ceramic strip holders use sample cups with ettan ipgphor manifold or ettan ipgphor manifold light to. Log files can be exported, for example to microsoft excel. Page 1 page 2 page 3 page 4 tools needed page 5 page 6 page 7 page 8 page 9 page 10 page 11 page 12 12.

Important user information please read this entire manual to fully understand the safe and effective use of this product. Cupbased sample application can improve proteinfocusing patterns, particularly in basic ipg strips, and the manifold accommodates either anodic or cathodic loading. The handbook will draw your attention to important safety related issues by care. Selective labelling of cellsurface proteins using cydye dige. The combination of ettan ipgphor 3 ief system, ettan. Introduction to estim erotic electrostimulation estim is yet another example of how technology is improving our lives. Ettan ipgphor 3 isoelectric focusing system function and description 1 ettan ipgphor 3 instrument user manual 11003458 edition ab control panel the ettan ipgphor 3 is controlled by a 7key membrane keypad. Ettan ipgphor 3 isoelectric focusing unit 11003364 unit to run first dimension ief ipgphor manifold, complete 80649838 for the most stringent applications.